Plasmid

Part:BBa_K4427016:Design

Designed by: Chanjuan Jin   Group: iGEM22_JLU_China   (2022-09-30)


Pet-22b-RLP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5536
    Illegal EcoRI site found at 8352
    Illegal XbaI site found at 5163
    Illegal XbaI site found at 5996
    Illegal XbaI site found at 8116
    Illegal PstI site found at 1140
    Illegal PstI site found at 6520
    Illegal PstI site found at 6817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5536
    Illegal EcoRI site found at 8352
    Illegal PstI site found at 1140
    Illegal PstI site found at 6520
    Illegal PstI site found at 6817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5536
    Illegal EcoRI site found at 8352
    Illegal BglII site found at 5097
    Illegal BglII site found at 9494
    Illegal BamHI site found at 6176
    Illegal XhoI site found at 10568
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5536
    Illegal EcoRI site found at 8352
    Illegal XbaI site found at 5163
    Illegal XbaI site found at 5996
    Illegal XbaI site found at 8116
    Illegal PstI site found at 1140
    Illegal PstI site found at 6520
    Illegal PstI site found at 6817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5536
    Illegal EcoRI site found at 8352
    Illegal XbaI site found at 5163
    Illegal XbaI site found at 5996
    Illegal XbaI site found at 8116
    Illegal PstI site found at 1140
    Illegal PstI site found at 6520
    Illegal PstI site found at 6817
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3317
    Illegal NgoMIV site found at 3477
    Illegal NgoMIV site found at 5065
    Illegal NgoMIV site found at 6127
    Illegal NgoMIV site found at 6162
    Illegal NgoMIV site found at 7410
    Illegal AgeI site found at 9971
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1


Source

q11

References